Coding

Part:BBa_K5057011

Designed by: Nafis Madappalli   Group: iGEM24_Freiburg   (2024-09-25)


SpC-P2

Construct coding for a fusion protein consisting of SpyCatcher [1] fused to the P2 phage tail protein [2], derived from the PRD1 bacteriophage. The construct also possess a HisTag at the very end to allow for easy protein purification. This fusion protein can be functionalized onto OMVs exhibiting SpyTag to act as targeting ligands, as part of our modular approach towards specificity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1065
    Illegal BamHI site found at 1008
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1102
    Illegal NgoMIV site found at 1384
    Illegal AgeI site found at 658
    Illegal AgeI site found at 846
    Illegal AgeI site found at 888
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1015


References

[1] Ghimire J, Hart RJ, Soldano A, Chen CH, Guha S, Hoffmann JP, et al. Optimization of Host Cell-Compatible, Antimicrobial Peptides Effective against Biofilms and Clinical Isolates of Drug-Resistant Bacteria. ACS Infectious Diseases [Internet]. 2023 Mar 24 [cited 2024 Sep 23];9(4):952–65.

[2] Huan Y, Kong Q, Mou H, Yi H. Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields. Frontiers in Microbiology [Internet]. 2020 Oct 16;11. ‌

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